Article provided by: bosterbio.com
The glyceraldehyde 3-phosphate dehydrogenase antibody or GAPDH antibody is an enzyme catalyzing the sixth step of glycolysis. A testis-specific isoenzyme GAPDH is even expressed. Bosterbio Antibody and ELISA offers kits and vials of the antibody with next day shipping.
What is the role of GAPDH in the body?
GAPDH is crucial to the breakdown of glucose into energy and carbon molecules. There is also evidence that the antibody aids in non-metabolic processes such as transcription activation, initiation of apoptosis, and fast axonal or axoplasmic transport.
The dehydrogenase catalyzes the conversion of glyceraldehyde 3-phosphate to D-glycerate 1,3-biphosphate, the sixth step in the breakdown of glucose. This conversion occurs in two coupled steps, with the second being unfavorable.
Role in transcription
GAPDH can activate transcription. Alongside the OCA-S transcriptional coactivator complex and lactate dehydrogenase, researchers now believe that the GAPDH is involved in more than metabolic processes.
Transcription may occur when the compound moves between the cytosol and nucleus. This causes a shift from the metabolic state to gene transcription.
Under oxidative stress, GAPDH acts as a metabolic switch. Cells require the cofactor NADPH in the presence of excessive amounts of oxidants.
Oxidant treatments cause the inactivation of GAPDH, which suppresses the generation of NADPH. The inactivation temporarily re-routes the metabolic flux from glycolysis to the Pentose Phosphate Pathway, allowing cells to generate more NADPH.
Does GAPDH effect more than the metabolic process?
This enzyme has multiple functions. Evidence implicates GAPDH in other cellular processes.
Research also suggests a role in maintaining cellular iron homeostasis. The enzyme acts as a chaperone protein for labile heme within cells.
How is GAPDH a factor in cancer?
GAPDH is overexpressed in human cancers, such as cutaneous melanoma. The expression positively correlates to tumor progression.
Glycolytic and antiapoptotic functions contribute to the proliferation and protection of tumor cells, promoting tumorigenesis. The enzyme inhibits the effectiveness of chemotherapeutic drugs by protecting against telomere shortening. Depleting GAPDH managed to induce senescence in tumor cells, presenting an opportunity to control growth.
GAPDH in Neurodegeneration
Neurodegenerative diseases and disorders also relate to the enzyme. GAPDH interacts with proteins specific to these conditions.
Interactions may affect metabolic and other GAPDH functions. Interactions with huntingtin may interfere with its function in apoptosis.
The antibody helps regulate the presence of GAPDH. Depleting the presence of the enzyme allows for NADPH production, which is beneficial in reducing high levels of oxidants.
Additionally, antibodies allow researchers to devise strategies to tackle a variety of cancers. Overexpression of GAPDH is a major component of these ontologies.
Where can I find GAPDH antibodies?
Bosterbio Antibody and ELISA offers quality and fast solutions. Researchers use our products in humans, mice, and rats to obtain significant results. More than 23000 publications cite our company as the source of their antibodies.
If you are researching metabolic processes or cancers, GAPDH antibodies are useful. Bosterbio offers next day shipping to help you dive into your research. Visit our website today for more information on our 100 microgram vials and ELISA kits.